RIBOPOOLS

riboPOOL

Affordable, Custom Ribosomal RNA Depletion for Any Species



Detection of scientifically relevant RNAs by Next-Generation RNA Sequencing (RNA-Seq) is greatly limited by highly abundant ribosomal RNAs (rRNAs) which occupy > 90% RNA-Seq reads. Current rRNA depletion solutions however are often costly and limited to well-studied species. 

Ribosomal RNA depletion pools (riboPOOLs) by siTOOLs Biotech is the market's most flexible solution in rRNA depletion. With a diverse selection of Ready-Made riboPOOLs and the ability to create your own Custom riboPOOL, riboPOOLs are ideal for sequencing experts with custom demands and scientists working on rare species.

Composed of high complexity pools of optimally-designed biotinylated DNA probes, riboPOOLs are highly efficient, even outperforming Ribo-Zero (Illumina), depleting ~10% more rRNA while leaving other RNA intact.

For microbiologists, the new Pan-Prokaryote riboPOOL provides universal rRNA depletion for a wide spectrum of microbes and is suited for metagenomic or microbiome samples. Combination riboPOOLs are also available to deplete rRNAs from samples containing multiple species. 

01 Highly efficient and specific

Human and mouse riboPOOLs depleted > 95% cytoplasmic and mitochondrial rRNA.

03 Low cost solution

A fraction of the price of existing solutions.

05 Applicable for non-polyadenylated RNA

riboPOOLs do not depend on polyA- enrichment enabling detection of non-polyadenylated RNA.

02 Any species

Deplete rRNA from any species of your choice. Human, Mouse and Planarian in-stock. New species available on demand.

04 Deplete custom RNA

Deplete any RNA of your choice, e.g. globin from whole blood samples.

06 Custom scales available

Larger scales available for custom application.

Supporting Data

Specific, efficient rRNA depletion with riboPOOLs

Human riboPOOL depleted 98.6% of rRNA, compared to 80.6% by Ribo-Zero Mouse riboPOOL depleted 96.4% of rRNA, compared to 86.1% by Ribo-Zero
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Reproducible results with riboPOOLs

High reproducibility between biological replicates with human riboPOOLs High reproducibility between biological replicates with mouse riboPOOls
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HOW IT WORKS

rRNA Depletion workflow

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Composed of a highly complex mixture of optimally-designed 3'-biotinylated DNA probes, riboPOOLs hybridize efficiently and specifically to ribosomal RNAs (rRNAs) present in total RNA. 

Streptavidin-coated magnetic beads are added that allow efficient separation of riboPOOL-bound rRNAs through the high affinity biotin-streptavidin interaction.

Remaining relevant RNAs are purified and concentrated by either ethanol precipitation, spin-column purification or SPRI bead-based clean-up, before analysis by Next-Generation Sequencing, microarray or PCR.