EV-Spinner: Ultrafiltration concentrator, MWCO 100 kDa, 48 pcs.

EV-Spinner is a 2.5 ml non-stick ultrafiltration (UF) concentrator to enhance the recovery of extracellular vesicle (EVs) during the concentration or buffer exchange step. The ultrafiltration works in the opposite direction to the centrifugal force, providing higher particle recovery. The low protein binding membrane (polyethersulphone) reduces the EV loss, compared to V-shape concetrators, and the reverse design of the EV-spinner ensures that the filter does not clog.

Applications: Concentration of solutions containing EVs, exosomes or nanoparticles. Concentration of diluted matrices as cell media or urine prior to EV isolation. Removal of small molecules and unbound dyes from solution containing EVs/exosomes and nanoparticles. EV and exosomes dialysis. Concentration of EV suspensions post SEC isolation.

Advantages: Non-stick, easy recovery of the retentate, washable, reusable multiple times.

Sample type

Fluids containing extracellular vesicles (EVs), exosomes and nanoparticles

Quantity

48 concentrators

Filter membrane

Polyethersulphone

Plastic construction

Polystyrene

Maximum sample volume

2.5 ml

Hold-up volume

0.050 ml

Centrifugation speed

2500 g Swing bucket rotor

2000 g Fixed angle rotor

Shelf life

24 months

Applications

Concentration of solutions containing EVs, exosomes or nanoparticles.

Concentration of diluted matrices as cell media or urine prior to EV isolation.

Removal of small molecules and unbound dyes from solution containing EVs/exosomes and nanoparticles.

EV and exosomes dialysis.

Concentration of EV suspensions post SEC isolation.