TFF-MV – tangential flow filtration-based device
Diluted EV containing matrices (i.e. urine, conditioned cell media)
TFF-MV is a filter cartridge in hollow fibers made of polysulfone. The filter is very useful for separating different EVs by size. Indeed, microvesicles bigger than 150 nm are retained inside the hollow fibers, while small EVs and molecules easily permeate the filter. Microvesicles can be recovered with a syringe in PBS buffer without additional purification steps.
Polysulfone hollow fibres
Sample volume per reaction
Recommended sample volume from 10 ml up to several liters if connected to mechanical pump.
From 5 minutes
Up to 20 times
Separation of EVs by size.
Recovering of big microvesicles in one step, without additional purification.