TFF-EVs-Small – tangential flow filtration-based device
EVs containing matrices (i.e. urine, conditioned cell media, plasma, serum)
Tangential flow filtration (TFF) is emerging as one of the most efficient methods for the purification of extracellular vesicles. The TFF-EVs filter allows a rapid, reproducible and scalable purification of EVs. It can be used on the lab bench for purifying small volume of samples or connected with a mechanical system for purifying larger volumes. During the purification, the sample is simultaneously concentrated.
Video guide: https://www.youtube.com/watch?v=D9gBxE6VtXc
Filter cartridge: Hollow fibers made of polyethersulfone
Filtering surface (sqm): 0.025
Fiber pore size (nm): 50 +/- 10
Cut off (kDa): 800
Sterilization method: e-beams sterilization
Sample volume per reaction: From 10 ml up to 1000 ml
EV recovery: 81%
Concentration time: 5 minutes
Reusable: Multiple times
Fast and scalable EV purification and concentration
• Depletion of FBS from bovine EVs
• Particle buffer exchange and removal of unbound dye.