TFF-EVs-Small- tangential flow filtration-based device

TFF-EVs-Small – tangential flow filtration-based device

Sample type:

EVs containing matrices (i.e. urine, conditioned cell media, plasma, serum)

Quantity:

1 concentrator

Product overview:

Tangential flow filtration (TFF) is emerging as one of the most efficient methods for the purification of extracellular vesicles. The TFF-EVs filter allows a rapid, reproducible and scalable purification of EVs. It can be used on the lab bench for purifying small volume of samples or connected with a mechanical system for purifying larger volumes. During the purification, the sample is simultaneously concentrated.

Video guide: https://www.youtube.com/watch?v=D9gBxE6VtXc

Technical Features:

Filter cartridge: Hollow fibers made of polyethersulfone

Filtering surface (sqm): 0.025

Fiber pore size (nm): 50 +/- 10

Cut off (kDa): 800

Sterilization method: e-beams sterilization

Sample volume per reaction: From 10 ml up to 1000 ml

EV recovery: 81%

Concentration time: 5 minutes

Reusable: Multiple times

Application:

Fast and scalable EV purification and concentration

• Depletion of FBS from bovine EVs

• Particle buffer exchange and removal of unbound dye.