TFF-EVs-Large- tangential flow filtration-based device

TFF-Easy – tangential flow filtration-based device

Sample type:

EVs containing matrices (i.e. urine, conditioned cell media, plasma, serum, plant extracts)

Quantity:

1 concentrator

Product overview:

Tangential flow filtration (TFF) is emerging as one of the most efficient methods for the purification of extracellular vesicles. The TFF-EVs filter allows a rapid, reproducible and scalable purification of EVs. It can be used on the lab bench for purifying small volume of samples or connected with a mechanical system for purifying larger volumes. During the purification, the sample is simultaneously concentrated.

Technical Features:

Filter cartridge: Polyethersulfone hollow fibres

Pore size: 50 nm

Cut off (kDa): 800

Filtering surface (m2): 1.0

Internal fiber diameter (μm): 210 +/- 10

External fiber diameter (μm): 290 +/- 20

Fiber number per filter : 6050 +/- 50

Cartridge internal diameter (mm): 32

Maximum transmembrane pressure (mmHg): 350

Maximum flow rate (ml/min):

Conditioned media 115ml/min
Urine 100ml/min
viscous fluids (plasma, serum) 78 ml/min

Sterilization method: e-beams sterilization

Sample volume per reaction: From 500 ml up to 10 000 ml

EV recovery: >80%

Concentration time:

78 ml/min-115 ml/min

Reusable:

Multiple times

Application:

Fast and scalable EV purification and concentration

• Depletion of FBS from bovine EVs

• Particle buffer exchange and removal of unbound dye.