mxPURE-EVs Size Exclusion Chromatography column + TFF-Easy concentrator + 1 TFF-MV concentrator
Urine, conditioned cell media or other diluted matrices
1 maxi column + 1 TFF-MV concentrator + 1 TFF-Easy concentrator
EVs can be effectively separated using a combination of double tangential flow filtration (TFF) and size exclusion chromatography (SEC).
TFF-MV allows to separate large microvesicles (>150 nm) from smaller vesicles. TFF-Easy allows to concentrate the remained matrix prior to EV isolation.
SEC column allows to isolate EVs from small molecules and ions in a fast and highly efficient way.
As a result, you get extracellular vesicles of a high purity in less than one hour.
Volume of sample:
5 ml – 20 ml (concentrated 10 folds by TFF-Easy)
Shipment and storage:
Extracellular vesicle isolation from diluted matrices (urine and cell culture media).
Purification of pre-isolated EVs from contaminants.