FLouEVs are a next generation of stably-fluorescent small EVs/Exosomes expressing the fluorescent protein EGFP (green) as fusion protein with the EV membrane marker CD63. Compared to the EVs labelled with fluorescent membrane dyes, FLouEVs demonstrated and increased stability of the fluorophore and a higher percentage of the fluorescent particles.
Characteristics: Particle size distribution 50 – 140 nm, lyophilized, storage at 4-8 °C.
Applications: Positive control for EV analyzers (NTA, flow Cytometers, fluorescence microscopy), cell spike-in and in vitro tracking, Ev uptake monitoring, assay and instrument calibration.
Lyophilized EGFP Fluorescent EVs from HEK293 cells (CD63-EGFP)
human embryonic kidney (HEK293 cells)
Quantity per vial
Storage of lyophilized exosomes
Store up to 1 years at 4°C (Expiration date indicated on the label).
Storage of reconstituted exosomes
Stored at -20°C for up to one month or at -80°C for up to 6 months. Recommended to avoid repeated freeze-and-thraw cycles.
NTA in fluorescent mode.
Cell spike- in and in vitro tracking.
Fluorescence assay calibration.