In its native state HA is normally present in the extracellular matrix as a high molecular weight, high viscosity polymer essential for maintenance tissue architecture, elasticity and hydration. However it also has other key functions including the regulation of cell behavior through specific interactions with cell surface receptors and extracellular proteins. HA binding to individual proteins commonly involves relatively short sequences in the HA polymer and there is considerable evidence that HA fragments generated in vivo have distinctive properties from the intact polymer.
Iduron offers a range of oligosaccharides produced by controlled endolyase scission of purified, low endotoxin Hyaluronic Acid (Streptococcal species). The oligosaccharides are separated by high resolution gel filtration and purity assessed on an analytical Superdex S75 HPLC column (see Data Sheets for profiles).
Size range of oligosaccharides dp2 to dp 20
dp is degree of monosaccharide polyimerisation. dp2 is a disaccharide, dp4 is a tetrasaccharide etc.
General formula: ∆HexAβ1,3 – [GlcNAcβ1,4 GlcAβ1,3]n – GlcNAc
n = number of disaccharide units
∆HexA is the C4-C5 unsaturated hexuronic acid at the non-reducing end of the oligosaccharides produced by endolyase scission of the HA polymer. The C4-C5 double bond absorbs strongly at 232nm and can be used for monitoring the oligosaccharides in various separation systems.
New Products – Biotin and Flourescently Labelled HA Oligosaccharides
In addition our HA Oligos we supply three biotin- or fluorescence-labelled (FITC or Ruthenium Red) hyaluronic acid (HA) oligomers with average mol wts of approximately 2200 (dp22), 2800 (dp28) and 3400 (dp34). The oligomers are tagged at their reducing ends to minimise interference with cell or protein interactions. A short PEG spacer is introduced between the biotin and the HA to facilitate use in SPR analyses. HA-oligosaccharides listed above (dp4 – dp20) may be available as labelled derivatives (biotin or fluorescence); please inquire at firstname.lastname@example.org