ARGO Library Preparation + riboPOOL for 24 rxn
$2,625.00
This product is both the ARGO RNA-seq Library Preparation Kit AND the riboPOOL depletion kit
The ARGO RNA-seq Library Preparation Kit uses a fragmentation-free, stranded, UMI-labelled workflow, optimized for both high-quality and degraded RNA samples, while being optimized for ribodepletion with our riboPOOLs.
RNA Library Preparation Kit with a fragmentation-free, stranded, UMI-labelled workflow for both intact (high-quality) and degraded RNA samples, while also being optimized and tested with our ribodepletion solution (riboPOOLs).
riboPOOL, nuclease-free water, streptavidin-coated magnetic beads, hybridization buffer, depletion buffer and ethanol precipitation reagents -linear acrylamide, clean-up beads and 3M sodium acetate
RNA Sequencing (RNA-Seq): Transcriptome Analysis & Gene Expression Profiling
What is RNA Sequencing?
RNA sequencing (RNA-Seq) is a next-generation sequencing (NGS) technology that captures and quantifies all RNA molecules in biological samples, delivering comprehensive transcriptome profiling and gene expression analysis. This genomics technique enables researchers to decode disease mechanisms, identify biomarkers, and discover therapeutic targets across cancer research, immunology, neuroscience, and precision medicine.
RNA-Seq Applications
- Differential gene expression (DGE): Quantify gene expression changes between conditions and disease states
- Alternative splicing detection: Identify splice variants, exon skipping, and isoforms
- Non-coding RNA profiling: Detect lncRNAs, circRNAs, microRNAs, and regulatory RNAs
- Variant calling: Discover SNPs, SNVs, indels, and mutations
- Gene fusion identification: Detect fusion transcripts in cancer genomics
- Pathway analysis: Map genes to biological pathways and networks
RNA Library Preparation for NGS
Exceptional RNA-Seq data begins with quality RNA library preparation. Advanced library prep kits convert fragile RNA to robust, sequencer-ready cDNA libraries for Illumina and other NGS platforms.
Modern workflows integrate unique molecular identifiers (UMIs), dual indexing, and strand-specific protocols—minimizing PCR bias, maximizing sequencing accuracy, and eliminating technical variability for reliable gene expression measurements.
Validated on a wide range of species
a)
b)
c)
Figure 1 Library profiles of a) Rattus norvegicus (rat), b) Lendenfeldia chondrodes (sponge) and c) Pinus sylvestris (pine tree) generated from 100 ng of total RNA depleted with siTOOLs Biotech riboPOOLTM rRNA-depletion kit. Bar plots show percentages of rRNA and RNA of interest in samples before and after ribodepletion with riboPOOLs.
| ARGO RNA Library Prep Kit Manual | Download |
|---|---|
| riboPOOL kit Manual | Download |
| rRNA depletion kit Brochure | Download |
| Download |
| Weight | 1 lbs |
|---|---|
| Dimensions | 18 × 13 × 2 in |
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