Narcissus pseudonarcissus lectin (NPA/NPL)

$935.00
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SKU:
05-0119-10mg

Larger Quantities Available Upon Request

Features 

  • Ultrapure quality
  • Sugar specificity: D-GalNAc
  • Miogenic activity
  • Non-reactive with human erythrocytes
  • Lyophilized powder

 

 

 

 

 

 

Product description

Narcissus pseudonarcissus lectin (NPL) is isolated from daffodils. It exists as a dimeric protein with the molecular weight of 26 kDa composed by two subunits of 13 kDa each. The lectin has specificity toward alpha linked mannose. NPL is used in characterisation of early stages of apoptosis and is mitogenic for human lymphocytes (1). The lectin agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes (2).

Medicago’s NPL lectin is supplied as a white lyophilized powder from a buffer containing 10 mM CH3COONH4. The purity is determined by SDS-PAGE, which generates one band at 13 kDa, corresponding to the two isomers (the lectin dissociates below pH 5.0 and above pH 9.0). The lectin is available in vials containing 50 mg or 10 mg lyophilized powder and the product is to be used for laboratory work only.

 

Applications

  • Mitogenic studies of human lymphocytes
  • Agglutination studies

 

Directions for use

The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol. The solution may be reconstituted in this buffer to desired working concentration.

 

Shipping and storage

The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than five years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.

Specifications

Appearance: White lyophilized powder or flocculate                              

Source: Daffodils

Molecular weight: 26 kDa 

Sugar specificity: D-GalNAc

Activity: Agglutinates rabbit erythrocytes at <0.1 μg/ml, inhibited by 10 mM D-galactose. NPL agglutinates both normal and trypsine treated rabbit erythrocytes.

Microorganisms: < 100 CFU/g

Protein content: > 90 %, OD280nm, Abs 280 nm (ε 1.35 = 1 mg/mL)

Identity: SDS-PAGE, one band at 13 kDa

Shelf life: > Five years when stored at -20°

 

References

(1) Summers C., Forrest J., Norval M., Sharp J. M. (2002) The potenitally insecticidal Narcissus pseudonarcissus lectin demonstrates age-related mitogenecity. FEMS immunology and medical microbiology vol 33 Issue 1, 47–9.

(2) Van Damme J. M., Allen A. K., Peumans W. J. (2007). Related mannose-specific lectins from different species of the family Amaryllidaceae. Physiologia Plantarum Vol 53, Issue 1, 52–7.

(3) Structure of Narcissus pseudonarcissus lectin complex with Mannobiose at 1.7 A resolution, form II Rizkallah, P.J., Ozbey, S., 
Sauerborn, M.K. To be Published. Where did we got the picture?