All riboPOOL probes in stock now on sale at 40% off till June 30th or I sell out. See the list below for details.



riboPOOLs - rRNA Depletion Kits

Detection of scientifically relevant RNAs by Next-Generation RNA Sequencing (RNA-Seq) is greatly limited by highly abundant ribosomal RNAs (rRNAs) which occupy > 90% RNA-Seq reads. Current rRNA depletion solutions however are often costly and limited to well-studied species. 

rRNA Depletion

riboPOOLs are high complexity pools of 3'-biotinylated single-stranded DNA.

riboPOOLs hybridize selectively to rRNA, enabling their removal using streptavidin-coated magnetic beads.



rRNA depletion workflow

Composed of a highly complex mixture of optimally-designed 3'-biotinylated DNA probes, riboPOOLs hybridize efficiently and specifically to ribosomal RNAs (rRNAs) present in total RNA.

Streptavidin-coated magnetic beads are added that allow efficient separation of riboPOOL-bound rRNAs through the high affinity biotin-streptavidin interaction.

Remaining relevant RNAs are purified and concentrated by either ethanol precipitation, spin-column purification or SPRI bead-based clean-up, before analysis by Next-Generation Sequencing, microarray or PCR.

Efficient, Affordable Ribosomal RNA Depletion for Any Species

Ribosomal RNA depletion pools (riboPOOLs) by siTOOLs Biotech is the market's most flexible solution in rRNA depletion. With a diverse selection of Ready-Made riboPOOLs and the ability to create your own Custom riboPOOL, riboPOOLs are ideal for sequencing experts with custom demands and scientists working on rare species.

Ribo-Zero Replacement

riboPOOLs offer the perfect replacement for Ribo-Zero , recently discontinued  by Illumina. The high complex pools of optimally-designed biotinylated DNA probes, outperforming Ribo-Zero (Illumina), depleting ~10% more rRNA while leaving other RNA intact.

Supporting Data

Specific, efficient rRNA depletion with riboPOOLs

Human riboPOOL depleted 98.6% of rRNA, compared to 80.6% by Ribo-Zero Mouse riboPOOL depleted 96.4% of rRNA, compared to 86.1% by Ribo-Zero
humanribopooldepeff.jpg mouseribopooldepeff.jpg
High reproducibility between biological replicates with human riboPOOLs High reproducibility between biological replicates with mouse riboPOOls
human-ribo-depletion-plots.jpg imgm-ribo-depletion-plots-mouse.jpg











Pan-Prokaryote riboPOOLs

For microbiologists, the new Pan-Prokaryote riboPOOL provides universal rRNA depletion for a wide spectrum of microbes and is suited for metagenomic or microbiome samples. Combination riboPOOLs are also available to deplete rRNAs from samples containing multiple species.

New Version 2 of Pan-Prokaryote


Quick links

Pan-Prokaryote riboPOOL Brochure

riboPOOL Poster