riboPOOLs are high complexity pools of 3'-biotinylated single-stranded DNA.
riboPOOLs hybridize selectively to rRNA, enabling their removal using streptavidin-coated magnetic beads.
rRNA depletion workflow
Composed of a highly complex mixture of optimally-designed 3'-biotinylated DNA probes, riboPOOLs hybridize efficiently and specifically to ribosomal RNAs (rRNAs) present in total RNA.
Streptavidin-coated magnetic beads are added that allow efficient separation of riboPOOL-bound rRNAs through the high affinity biotin-streptavidin interaction.
Remaining relevant RNAs are purified and concentrated by either ethanol precipitation, spin-column purification or SPRI bead-based clean-up, before analysis by Next-Generation Sequencing, microarray or PCR.
Efficient, Affordable Ribosomal RNA Depletion for Any Species
Ribosomal RNA depletion pools (riboPOOLs) by siTOOLs Biotech is the market's most flexible solution in rRNA depletion. With a diverse selection of Ready-Made riboPOOLs and the ability to create your own Custom riboPOOL, riboPOOLs are ideal for sequencing experts with custom demands and scientists working on rare species.
riboPOOLs offer the perfect replacement for Ribo-Zero , recently discontinued by Illumina. The high complex pools of optimally-designed biotinylated DNA probes, outperforming Ribo-Zero (Illumina), depleting ~10% more rRNA while leaving other RNA intact.
For microbiologists, the new Pan-Prokaryote riboPOOL provides universal rRNA depletion for a wide spectrum of microbes and is suited for metagenomic or microbiome samples. Combination riboPOOLs are also available to deplete rRNAs from samples containing multiple species.