RIBOPOOLS (RNA depletion)



riboPOOLs - rRNA Depletion Kits

Detection of scientifically relevant RNAs by Next-Generation RNA Sequencing (RNA-Seq) is greatly limited by highly abundant ribosomal RNAs (rRNAs) which occupy > 90% RNA-Seq reads. Current rRNA depletion solutions however are often costly and limited to well-studied species. 

rRNA Depletion

riboPOOLs are high complexity pools of 3'-biotinylated single-stranded DNA.

riboPOOLs hybridize selectively to rRNA, enabling their removal using streptavidin-coated magnetic beads.



rRNA depletion workflow

Composed of a highly complex mixture of optimally-designed 3'-biotinylated DNA probes, riboPOOLs hybridize efficiently and specifically to ribosomal RNAs (rRNAs) present in total RNA.

Streptavidin-coated magnetic beads are added that allow efficient separation of riboPOOL-bound rRNAs through the high affinity biotin-streptavidin interaction.

Remaining relevant RNAs are purified and concentrated by either ethanol precipitation, spin-column purification or SPRI bead-based clean-up, before analysis by Next-Generation Sequencing, microarray or PCR.

Efficient, Affordable Ribosomal RNA Depletion for Any Species

Ribosomal RNA depletion pools (riboPOOLs) by siTOOLs Biotech is the market's most flexible solution in rRNA depletion. With a diverse selection of Ready-Made riboPOOLs and the ability to create your own Custom riboPOOL, riboPOOLs are ideal for sequencing experts with custom demands and scientists working on rare species.

Ribo-Zero Replacement

riboPOOLs offer the perfect replacement for Ribo-Zero , recently discontinued  by Illumina. The high complex pools of optimally-designed biotinylated DNA probes, outperforming Ribo-Zero (Illumina), depleting ~10% more rRNA while leaving other RNA intact.

Supporting Data

Specific, efficient rRNA depletion with riboPOOLs

Human riboPOOL depleted 98.6% of rRNA, compared to 80.6% by Ribo-Zero Mouse riboPOOL depleted 96.4% of rRNA, compared to 86.1% by Ribo-Zero
humanribopooldepeff.jpg mouseribopooldepeff.jpg
High reproducibility between biological replicates with human riboPOOLs High reproducibility between biological replicates with mouse riboPOOls
human-ribo-depletion-plots.jpg imgm-ribo-depletion-plots-mouse.jpg


Pan-Prokaryote riboPOOLs

For microbiologists, the new Pan-Prokaryote riboPOOL provides universal rRNA depletion for a wide spectrum of microbes and is suited for metagenomic or microbiome samples. Combination riboPOOLs are also available to deplete rRNAs from samples containing multiple species.

Ready-made riboPOOLs are available for diverse species.

riboPOOLs for Eukaryotes riboPOOLs for Prokaryotes riboPOOLs for Abundant RNA
Homo sapiens (human) 001
Mus musculus (mouse) 002
Saccharomyces cerevisiae 005
Filamentous fungi 006
Drosophila melanogaster (fruit fly) 007
Arabidopsis thaliana 008
Oryza sativa (rice) 009
Danio rerio (Zebrafish) 010
Amphimedon queenslandica 011
Chinchilla lanigera (rodent) 014
Loripes orbiculatus & Lucinoma aequizonata 017
Pichia pastoris 019
Plautia stali 020
Ustilago maydis 024
Schmidtea mediterranea (planarian flatworm) 028
Bombyx mori (Silkworm) 029
Lxodes scapulars (ticks) 030
Pan Plant (Angiospermae) 031
Pan Bird 032
Emiliania huxleyi 033
PolyA 034
Pan Prokaryote (Universal Microbe) 003
Escherichia coli 004
Bacillus subtilis 012
Caulobacter crescentus 013
Clostridium perfringens 015
Haloferax volcanii 016
Pseudomonas aeruginosa 018
Staphylococcus aureus 021
Salmonella enterica 022
Stenotrophomonas sp 023
Pan Bacteria 026
Pan Archaea 027
Pan Actinobacteria 035
Human Globin 025



 decisiontree-ribopools.pngQuick links

Pan-Prokaryote riboPOOL Brochure

riboPOOL Poster