ExoTEST™ Ready to Use Kit for Overall Exosome capture and quantification from Cell culture supernatant (white plate, with lyophilized exosomes from COLO1 cells)

$868.00
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SKU:
HBM-RTK-POC/WC

ExoTEST™ is a sandwich enzyme-linked immunoassay for in-vitro exosome quantification in cell culture supernatant.

 

Sample type

Cell culture supernatant.

Starting material

Up to 100 µL of biological sample can be loaded per well.

Concentrated cell culture supernatant samples are recommended prior capture according to our suggested protocol.

Assay type

Sandwich enzyme-linked immunoassay.

Sensitivity

The detection limit of an assay is lower than 0.35 μg of overall exosome which is an equivalent of less than 50 pg of targeted exosomes protein.

Product overview

ExoTEST™ Ready to Use Kit is the ideal platform for capturing and the quantifying exosomes from cell culture supernatant.

ExoTEST™ Ready to Use Kit features pre-coated ELISA plate with primary antibodies detection directed against exosome-specific markers, namely α-CD9. Secondary antibodies and substrate reagents are included in the kit.

Exosome standards for assay calibration are also included in the kit. Exosome standards are purified by ultracentrifugation and microfiltration, quantified for overall protein content and exosome number and then lyophilized for long-term storage.

ExoTEST™ ready to use kits are reliable, easy to use, works on a variety of commercially available detection technologies.

Primary antibody

α-CD9 biotinylated murine antibody.

Exosome standard

Lyophilized exosomes from COLO-1 Cell culture supernatant (see details).

Assay protocol

Kit contains all the reagents necessary for exosome capture and protein targeted detection (see Product Insert)

Assay calibration: Kit contains exosome standards prepared from purified from proprietary human cell line for assay calibration. All exosome standards are lyophilized and quantified for overall protein content and exosomes number.

HansaBioMed also provides a range of exosome standards purified from different types of human cells that can be purchased separately or included in custom kits in order to meet the need for detection of specific exosome markers.

Shipment and storage

All the reagent are shipped at 4°C and storage conditions as recommended in the product insert.

Expiration date

12 months.

 

 

Application :

Exosome capture and quantification using the ExoTEST™ ensures dose-dependent exosome detection in a variety of samples. The quantification can be both relative and absolute as the assay calibration enables the calculation of the approximate exosome quantity for each sample.

Comprehensive exosome profiling by implementing different combinations of capture and detection antibodies. ExoTEST™ can be customized for assessing multiple antigens in a total exosome population within a sample. Informative protein profiles for the samples of interest can be combined with subsequent nucleic acid (mRNA or miRNA) extraction and downstream analysis from captured exosomes.

Specific diagnostic applications using the ExoTEST™ allows the quantitative assessment of total circulating exosomes and specific exosomal sub-populations, defined by differentially expressed antigens under normal and pathological conditions.

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