Microvesicles (150- 1000 nm) are separated by tangential flow filtration using the TFF-MV filter, then purified by SEC. Isolated vesicles are quantified and validated for overall protein content, size distribution and particle number by NTA (Nanoparticles Tracking Analysis) with Zetaview analyzer (Particle Metrix). Effects of lyophilization on stability of EV proteins are comparable to other storage methods such as storing fresh exosomes at -20C. Lyophilized exosomes are easy to store and ship and are stable for long term (up to 36 months).