Aleuria aurantia lectin (AAL)

$186.00
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SKU:
05-0134-2mg

2mg size-Larger Quantities Available Upon Request

 

Features 

  • Ultrapure quality
  • Sugar specificity: fucose and terminal fucose residues on complex oligosaccharides and glycoconjugates
  • Binding affinity for fucose in all binding positions (α1-2, α1-3, α1-4 and α1-6)
  • Higher affinity towards fucosylated oligosaccharides than native AaL
  • Not blood group specific 

 

 

 

 

Product description

Recombinant Aleuria aurantia lectin is produced in E.coli and has an amino acid sequence identical to native Aleuria aurantia lectin. AAL is a dimeric lectin with two identical subunits of approximately 36 kDa. Each subunit has five carbohydrate-binding sites (1). The lectin recognizes and binds specifically to fucose and terminal fucose residues on complex oligo saccharides and glycoconjugates. rAAL has binding affinity for fucose in all binding positions (α1-2, α1-3, α1-4 and α1-6) and in contrast to AAL purified from natural sources, rAAL is not contaminated with free fucose yielding higher affinity towards fucosylated oligosaccharides than native AAL (2).

Recombinant AAL hemagglutinates erythrocytes irrespective of blood type (A, B and 0) at the same titers as AAL isolated from natural sources.

AAL has been widely used for analysis and preparation of oligosaccharides and glycoproteins (3). Diagnostic applications include analysis of disease-associated glycosylation on plasma proteins (4). Furhtermore, rAAL can be immobilized and used for affinity chromatography (5).

 

Applications

  • Studies of glycoproteins and glycolipids
  • Purification of membrane proteins
  • Affinity chromatography
  • Agglutination studies

 

Directions for use

The lectin may be reconstituted with 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08 % sodium azide, 0.1 mM Ca++, 0.01 mM Mn++. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol.

 

Shipping and storage

The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with HEPES buffer, the solution may be stored frozen in working aliquots for up to 12 months.

 

Specifications

Appearance: White to pale yellow lyophilized powder or flocculate

Source: E.coli

Molecular weight: Two identical subunits of 36 kDa

Sugar specificity: Fucose α1-2, α1-3, α1-4 and α1-6

Activity: Agglutinates human erythrocytes irrespective of blood type (A, B and 0).

Microorganisms: < 100 CFU/g

Protein content: > 80 %

Identity: SDS-PAGE, 4 mg/ml, staining with coomassie R-350, one band between 30 and 45 kDa

Shelf life: > Five years when stored at -20°